商品编号


2350



品牌


Sciencell



公司


Sciencell research lab



公司分类


Human Pluripotent Stem Cells




1 Ea

商品信息
Isolated from fetal human skin. HDF-f-mt are cryopreserved at P0 and delivered frozen. Each vial contains > 5 x 10^5 cells in 1 ml volume.

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their dur
ABI
lity also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen [1]. There is evidence showing that fibroblasts in various organs are intrinsically different [2]. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli [3].
HDF-f-mt from
Sciencell
Research Laboratories are isolated from fetal human skin. HDF-f-mt are cryopreserved at P0 and delivered frozen. Each vial contains > 5 x 10^5 cells in 1 ml volume. HDF-f-mt are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HDF-f-mt are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HDF-f-mt are guaranteed to further culture under the conditions provided by
Sciencell
Research Laboratories; however, HDF-f-mt are not recommended for expanding as mitomycin C treatment prevents further cell proliferation.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HDF-f-mt in vitro.

Specifications:
Catalog No.
2350
Country of Manufacture
United States
Product Code
HDF-f-mt
Size/Quantity
1 x 10^5 cells/vial
Product Use
HDF-f-mt are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping Info
Dry ice.
References
[1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea,heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137. [2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.[3] Stair S, Carlson KW, Shuster S, Wei ET, Stern R (2002) Mystixin peptides reduce hyaluronan deposition andedema formation. Eur J Pharmacol 30;450(3):291-6.