商品编号


SC038-GP



品牌


Gentarget



公司


Gentarget



公司分类


Premade stable cells




1 Ea

商品信息

Transformed from Hela cells?via lentiviral system, expressing the rtTA (reverse tetracycline Transcriptional Activator) protein.
?
rtTA-m2
(
ABC65845.1
) is constitutively?expressed under the enhanced EF1a promoter. A
GFP
-Puromycin
?fusion dual
Marker
was expressed under RSV promoter (see the integrated expression cassette below). Click ?
Product Manual
?for more details
.

?

This cell line expressed rtTA protein binds to tetracycline operator elements (TetO) in the presence of doxycycline (Dox). Any promoters that embedded with TetO sequences can binds to the rtTA protein when the inducer (Dox or tracycline) is present, which activates the transcription of the promoter’s downstream gene (So called,
T
et-O
n

induc
IBL
e system). Therefore, this cell line can be used for Tetracycline or Dox induc
IBL
e target expression when applied with target expression vectors (with TetOn induc
IBL
e promoter), or for generation of any induc
IBL
e target stable cell lines (after introduced a TetOn expression vector).

Sold at:? 1 vial x (>2?x 10
6
cells)/vial
?

Cat# SC038-GP