商品编号


0504-100



品牌


Reachbio



公司


Reachbio



公司分类


Human Peripheral Blood Cells




15

商品信息

Product Description

Human Peripheral Blood CD4
+
Helper T Cells





Catalog

Number



Selection

Type



Unit Size

(cells per vial)


Product

Info Sheet



0504-100

Negative

15 million

download




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Cell Enrichment

Negative Selection

CD4
+
Helper T Cells are enriched from human peripheral blood (PB) mononuclear cells (MNCs) using a negative selection immunomagnetic separation method involving of an antibody cocktail that targets and depletes non-CD4
+
cells.

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Sample Collection and Processing
Peripheral blood cells are collected from normal human volunteers via leukapheresis (COBE
?
Spectra MNC collection protocol or equivalent). The mononuclear cell-rich leukocyte layer (leukapheresis product) is continually aspirated by a low flow rate collection pump directly into a collection bag. Immunomagnetic cell enrichment of CD4
+
cells is? performed within 30 hours of collection. Donors have been screened and found to be negative for a standard panel of infectious agents that at minimum includes HIV, HBV and HCV. Depending on the specific lot, other infectious agents may also have been tested for (Certificate of Analysis includes lot-specific testing information). ?
Purity
Purity of CD4
+
Helper T Cells is >90% as measured by flow cytometric analysis.
?

St
ABI
lity and Storage
Peripheral blood CD4
+
cells are cryopreserved in CryoStor? CS5, a serum-free freezing medium. Cells are stable for long-term storage at -152
o
C or in the gaseous phase of liquid nitrogen. Storage at -80
o
C is not recommended. Once thawed, the cells must be used immediately and have a finite life span in culture.
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Intended Use
FOR IN VITRO RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR VETERINARY USE IN VIVO. NOT TO BE USED FOR ANY DIAGNOSTIC, THERAPEUTIC OR CLINICAL APPLICATIONS WHATSOEVER. ?
Additional Information



Weight

1 lbs



Species

Human




Tissue Source

Peripheral Blood




Cell Type

T Cells, T Cells – Helper




Selection Type

Negative




Identifying Surface
Marker

CD4




Unit Size (million cells/vial)

15




Format

Cryopreserved