商品编号


72033



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Cell Based Assay Kits



Size

100 reactions

商品信息













Background:
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3 dioxygenase (TDO), one of the rate limiting enzymes in this pathway, is upregulated in many tumors, providing cancer cells with an avenue for immune evasion.




Description:
The
human TDO Cell-Based Assay Kit
is designed to monitor the activity of exogenously expressed
human TDO
in cultured cells.




Synonym(s):
Tryptophan 2,3-dioxygenase, TDO2, TDO-2, TDO




Supplied As:
The kit contains a transfection-ready expression vector for full length human TDO (hTDO). Upon expression, hTDO can convert L-Trp to Kyn, which gets released and is detected in the assay medium. The kit also includes specialized assay medium.




Format:

COMPONENTS:





Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Screen for inhibitors of hTDO enzyme in a cellular context.




Reference(s):
1. Yue, E.,
et al., J. Med. Chem
. 2009;
52
: 7364–7367.
2. Liu, X.,
et al., Blood
. 2010;
115(17)
: 3520-3530.




Warning(s):
Avoid freeze/thaw cycles.




Product Type:
Assay Kit




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for specific information on other lots.