商品编号


60689



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Immunotherapy



Size

2 vials

商品信息













Background:
SIRP alpha (CD172A, PTPNS1, SIRP a) is a member of the signal-regulatory-protein (SIRP) family, which are transmembrane immunoglobulin receptors that negatively regulate receptor tyrosine kinase-coupled signaling processes. SIRP alpha is expressed predominantly on macrophages and dendritic cells. Interaction with its ligand CD47 mediates signals that blocks phagocytosis (also known as the “don’t eat me” signal).




Description:
Recombinant HEK293 cell constitutively expressing full length human SIRPa (#NM_080792). Surface expression is confirmed by flow cytometry.




Synonym(s):
sirp hek293, sirp a, sirpa, SIRPα cell line, SIRP cell line, CD47 cell line , sirp alpha sirpa




Host Cell line:
HEK293




Mycoplasma Testing:

This cell line has been screened using the (Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Cat # LT07-518) was used as a positive control.




Growth Media:

Thaw Medium 1 (BPS Bioscience, Cat. #60187):
MEM medium (Hyclone, Cat. #SH30024.01) supplemented with 10% FBS (
Invitrogen
, #26140-079), 1% non-essential amino acids (Hyclone, Cat. #SH30238.01), 1 mM Na pyruvate (Hyclone, Cat. #SH30239.01), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01)

Complete Growth Medium:
Thaw Medium 1 (BPS Bioscience, Cat. #60187) plus 2 ?g/ml Puromycin Dihydrochloride (
Thermo
Fisher, Cat. #A1113803).




Format:
Each vial contains ~2 x 10^6 cells in 1 ml of 10% DMSO in FBS.




Storage / St
ABI
lity:
Store in liquid nitrogen immediately upon receipt.




Application(s):
This cell line is useful for SIRPα:CD47 protein binding analyses and screening for antibodies or inhibitors of CD47.




Reference(s):
1. Lee WY et.al. (2010) The Role of cis Dimerization of Signal Regulatory Protein a (SIRPa) in Binding to CD47. J. Biol. Chem. 285: 37953-37963




Notes:

Contact us for pricing.

License Disclosure
:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suit
ABI
lity of the cell line for any particular use, and does not accept any li
ABI
lity in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bp
SBI
oscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.




Scientific Category:
Immunotherapy




Product Type:
Cell Line




Data shown is lot-specific.
Contact us
for specific information on other lots.