商品编号


90331



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Cell Surface Receptor(s)



Size

2 vials

商品信息





Species: Human





Genbank #: NM_003307





Background:
TRPM2 channel belongs to the transient receptor potential channel (TRP) superfamily which is divided into seven main subfamilies. TRPM2 channel is a nonselective, calcium-permeable cation channel highly expressed in the brain, vascular smooth muscle, endothelial cells and immune cells. It is activated by oxidative stressors such as hydrogen peroxide, cyclic ADP ribose, and NADP, le
ADI
ng to an increase in the intracellular free calcium concentration and cell death. TRPM2 is a potential drug target in a variety of human diseases, including cardiovascular and neurodegenerative diseases.




Description:
Stable, recombinant HEK293 cell line expressing tetracycline-induc
IBL
e human TRPM2 (transient receptor potential cation channel, subfamily M, member 2, accession number NM_003307).




UniProt
O94759




Synonym(s):
transient receptor potential cation channel, subfamily M, member 2, TRPM2, KNP3, previously TRPC7




Supplied As:
Each vial contains ~1.5 X 10
6
cells in 1 ml of 10% DMSO.




BIOLOG
ical Activity:
N-terminal FLAG tagged human TRPM2 channel has been stably integrated into HEK293 cells and its expression can be induced by tetracycline (doxycycline). The tetracycline-induc
IBL
e expression of TRPM2 was confirmed by Western blotting. The function of TRPM2 was characterized by calcium assay. TRPM2-HEK293 cells were pre-treated with doxycycline to induce the expression of TRPM2, then stimulated with H
2
O
2
, and calcium influx was observed. The H
2
O
2
-induced calcium influx via TRPM2 could be blocked by N-(p-Amylcinnamoyl) anthranilic Acid (ACA), a TRP channel blocker.




Host Cell line:
HEK293 cells




Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.




Instructions for use:
See cell line data sheet for detailed culturing and assay protocol.




Storage / St
ABI
lity:

Store in liquid nitrogen immediately upon receipt.




Application(s):
?Monitor TRPM2 calcium channel activity
?Screen for activators or inhibitors of TRPM2 calcium channel




Reference(s):
1. Moran, M.M., et al. (2011). Nat. Rev. Drug Discov. 10(8):601-620
2. Song, Y., et al. (2008) J. Biomol. Screen. 13(1):54-61
3. Fonfria, E., et al. (2004). Br. J. Pharmacol. 143(1):186-192.




Notes:

Contact us for pricing.

License Disclosure
:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suit
ABI
lity of the cell line for any particular use, and does not accept any li
ABI
lity in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bp
SBI
oscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Ion Channel




Product Type:
Cell Line




Data shown is lot-specific.
Contact us
for specific information on other lots.