商品编号


3710-001-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


Organoid and Stem Cell Research



Size

1 Vial (0.5 ml), 1x10^6 cells

商品信息

Description

NOTE
:?Starting January 1st, 2017 free sample sizes of?Cultrex??Organoid Harvesting Solution,?Cultrex? BME Type 2, ?and?Cultrex? BME Type R1 ?will be added to your order. ?These products are also sold individually, more information on
Trevigen
?’s Cultrex Organoid products can be found here.

Roof plate-specific Spondin-1 (R-Spondin 1 or Rspo1), also known as CRISTIN3, is a 27 kDa secreted activator protein that belongs to the R-Spondin family. R-Spondins positively regulate Wnt/beta-catenin signaling, most likely by acting as a ligand for LGR4-6 receptors and an inhibitor for ZNRF3. R-Spondin-1 induces proliferation of intestinal crypt epithelial cells, increases intestinal epithelial healing, and supports intestinal epithelial stem cell renewal.

Features:


Robust and stable expression of R-Spondin1.

Two epitope tags provide flexibility to purify and detect the expressed protein.

Acces
IBL
e source of either purified R-Spondin1 or R-Spondin1 conditioned medium.

Suitable to supplement both human and murine organoid culture medium.


Quality control specifications:


Negative by PCR for Mycoplasma.

Expression detected by Western Blot – A band of approximately 70 kDa corresponding to HA-R-Spondin1-Fc is detected in the conditioned medium of cells after 7 days of culture.

BIOLOG
ical activity determined for each lot:

TopFlash assay – HA-R-Spondin1-Fc must induce the expression of luciferase under the control of a TCF/LEF promoter.