商品编号


3520M-4APW-10



品牌


Mabtech



公司


Mabtech



公司分类


NHP



Size

10 plates














商品信息
ELISpot
PLUS
for enumeration of cells secreting IL-17A
This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting monkey IL-17A. The kit includes ELISpot plates pre-coated with monoclonal antibody, biotinylated detection antibody, Streptavidin-ALP and ready-to-use BCIP/NBT-plus substrate. The included anti-CD3 mAb can be used to induce cytokine secretion and is dependent on number, nature and vi
ABI
lity of accessory cells. The antibody cross-reacts with CD3 on cynomolgus and rhesus macaque T cells.
The monoclonal antibodies in this kit react with human IL-17A and cross-react with IL-17A from non-human primates See the Human/NHP cross-reactivity table.
Pre-coated plates reduce assay time and minimize vari
ABI
lity.
Monkey IL-17A ELISpot
PLUS
kits are also available with Streptavidin-HRP and TMB substrate. See Related products.

Analyte Information

IL-17A
Interleukin-17A (IL-17A, IL17A) is a potent proinflammatory cytokine produced by activated Th17 (T-helper 17) cells and certain cells belonging to the innate immune system. In mice, IL-17 has been shown to also be produced by activated CD8+ T cells and γδ T cells. Th17 cells play an important role in autoimmune diseases and protection against bacteria and fungi. IL-17A acts on a broad range of cell types to induce expression of cytokines, chemokines and metalloproteinases. As a result, secretion of IL-17A promotes inflammatory responses, which leads to the recruitment of neutrophils, enhancement of antibody production and activation of T cells. Increased expression of IL-17A is seen in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. It is also associated with asthma, psoriasis, cancer and transplant rejection.
Application:


ELISpot


Reactivity:


NHP




Contents:


Biotinylated detection mAb (MT504)
Streptavidin-ALP
anti-CD3 mAb
Substrate (BCIP/NBT-plus)
10
Pre-coated white plates (mAb MT241)


Size:

10 plates

Other formats:

Click to view all NHP IL-17Aproducts






I have compared
Mabtech
ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples.
Mabtech
's kits proved to be superior
to our protocol, had significantly lower background and provided better and higher detection of spots.








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Mabtech/Monkey IL-17A ELISpot
PLUS
(ALP)/3520M-4APW-10/10 plates






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