商品编号


52002L



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Methyltransferase(s)



Size

96 reactions

商品信息













Background:
PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3.




Description:
The PRMT5 Direct Activity Chemiluminescent Assay Kit is designed to measure PRMT5 activity for screening and profiling applications. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.




Synonym(s):
PRMT5, HRMT1L5, IBP72, JBP1, SKB1




Supplied As:
Kit comes in a convenient format, with a 96-well plate precoated with histone H4 peptide substrate, reagents, and purified PRMT5 enzyme for 100 enzyme reactions.




Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt




Format:

COMPONENTS:






Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.




Reference(s):
Dillon S.C., et al. 2005. Genome
BIOLOG
y 6:227.




Notes:
Since May of 2017, this kit has been improved with a more specific antibody and reformulated buffers. The previous version of PRMT kit #52002L can still be purchased upon special request.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Methyltransferase




Product Type:
Assay Kit




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