商品编号


52058



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Methyltransferase(s)



Size

384 reactions

商品信息













Background:
PRMT8 is a histone methyltransferase that exhibits methylation activity toward histones H2A and H4.




Description:
The PRMT8 Homogeneous Assay Kit is designed to measure the activity of PRMT8 for screening and profiling applications, using AlphaLISA
?
technology. The key to the PRMT8 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT8 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by re
ADI
ng the Alpha-counts.




Supplied As:
The PRMT8 Homogeneous Assay Kit comes in a convenient AlphaLISA
?
format, with a 384-well plate, biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT8 for 384 enzyme reactions.




Contraindications:
Avoid green and blue dyes that absorb light in the Alphascreen
?
signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN
3
) or metal ions (Fe
2+
, Fe
3+
, Cu
2+
, Zn
2+
and Ni
2+
). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen
?
assays.




Format:






Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.




Reference(s):
Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Methyltransferase




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.