商品编号


52060



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Methyltransferase(s)



Size

96 reactions

商品信息













Background:
SETD2 is a histone methyltransferase that exhibits methylation activity toward histones H3-K36




Description:
The SETD2 Direct Activity Chemiluminescent Assay Kit is designed to measure SETD2 activity for screening and profiling applications. The key to the SETD2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.




Synonym(s):
SET Domain Containing 2, HYPB1, HIF-1, KMT3A, KIAA1732, SETD2




Supplied As:
Kit comes in a convenient strip format, with a 96-well plate precoated with histone H3 peptide substrate, purified SetD2 and reagents for 100 enzyme reactions.




Contraindications:
Avoid green and blue dyes that absorb light in the Alphascreen
?
signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN
3
) or metal ions (Fe
2+
, Fe
3+
, Cu
2+
, Zn
2+
and Ni
2+
). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen
?
assays.




Format:





Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.




Reference(s):
Dillon S.C., et al. 2005. Genome
BIOLOG
y 6:227.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Methyltransferase




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.