Bpsbioscience/EZH2-EED Binding Assay Kit/52066/384 reactions
其他试剂盒
商品编号
52066
品牌
Bpsbioscience
公司
BPS Bioscience Inc.
公司分类
Methyltransferase(s)
Size
384 reactions
商品信息
Background:
EZH2 is a histone methyltransferase that exhibits methylation activity toward H3-K27
Description:
The EZH2-EED Binding Assay Kit is designed to measure the inhibition of the
protein-protein interaction between EZH2 and EED. The key to this kit is the specific binding of EZH2 to EED. With this kit, only two
simple steps on a microtiter plate are required. First, incubate a sample containing EZH2, EED
and an inhibitor of choice. Next, incubate with a mixture of acceptor and donor beads, followed
by re
ADI
ng the Alpha-counts.
Synonym(s):
KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH
Supplied As:
The kit comes in a convenient AlphaLISA? format, with GST-tagged EZH2, FLAG-tagged EED, and enough assay buffer to perform a total of 384 reactions.
Assay Conditions:
Assay was done using AlphaLISA format to measure the inhibition of the protein-protein interaction between EZH2 and EED. Reaction was performed at room temperature for 1 hour. Anti-FLAG Acceptor Beads and Glutathione Donor Beads were added and the reaction was incubated for 1 hour at room temperature. Alpha Counts were read on AlphaScreen microplate reader.
Format:
COMPONENTS:
Instructions for use:
See product datasheet for detailed assay protocol.
Storage / St
ABI
lity:
At least one year from date of receipt when stored as directed.
Application(s):
Useful for the study of EZH2 binding assays, screening inhibitors and selectivity profiling.
Reference(s):
Kong, X.,
et al., J. Med. Chem
. 2014;
57:
9512.
Notes:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Anti-FLAG AlphaLISA? Acceptor Beads Beads, 5 mg/ml (
Perkinelmer
#AL112C)
AlphaScreen? Glutathione Donor Beads, 5 mg/ml (
Perkinelmer
#6765300)
Optiplate-384 (
Perkinelmer
#6007290) AlphaScreen? microplate reader
Adjustable micropipettor and sterile tips
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase
Product Type:
Assay Kit
Data shown is lot-specific.
Contact us
for specific information on other lots.
产品货号:15580.0