商品编号


60384



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Phosphodiesterase(s)



Size

384 reactions

商品信息













Background:
Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4C selectively hydrolyzes cAMP and is a candidate bio
Marker
for human glioma. Research suggests that PDE4C functions as a tumor suppressor, and downregulation of PDE4C in glioma patients correlates with poor prognosis.




Description:
The PDE4C1 Assay Kit is designed for identification of PDE4C1 inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4C1 to the binding agent. The key to the PDE4C1 Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4C1 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4C1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.




Supplied As:
The PDE4C1 inhibitor screening assay kit comes in a convenient 384-well format, including purified PDE4C1 enzyme, fluorescently labeled PDE4C1 substrate (cAMP), binding agent, and PDE assay buffer for 400 enzyme reactions.




Format:

COMPONENTS:






Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.




Reference(s):
Chandrasekaran, A.,
et al., Cell Signal
. 2008;
20(1)
:139-53.




Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable to measure fluorescence polarization




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Phosphodiesterase




Product Type:
Assay Kit




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for specific information on other lots.