商品编号


80580



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


PARP(s)



Size

96 reactions

商品信息













Description:
The PARP1 Colorimetric Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP1 Colorimetric Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.




Synonym(s):
poly(ADP-ribose)polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1




Supplied As:
The PARP1 assay kit comes in a convenient 96-well format, with purified PARP1 enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 100 enzyme reactions.




Format:

COMPONENTS:





Instructions for use:
See assay kit data sheet for detailed protocol




Storage / St
ABI
lity:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.




Reference(s):

1.Brown, J.A., Marala, R.B. 2002.
J. Pharmacol. Toxicol. Methods
47:
137-41.

Application Reference(s):
1.?
Olaparib hydroxamic acid derivatives as dual PARP and HDAC inhibitors for cancer therapy
(2017)




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
PARP




Product Type:
Assay Kits




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