商品编号


51031



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Methyltransferase(s)



Size

96 reactions

商品信息













Description:
The SMYD2 Chemiluminescent Assay Kit is designed to measure SMYD2 activity for screening and profiling applications. The key to the SMYD2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes a methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and the SMYD2 methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.




Synonym(s):
KMT3C




Supplied As:
The SMYD2 Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 substrate, antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase




Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt




Format:

COMPONENTS:






Instructions for use:
See product datasheet for detailed assay protocol.




Storage / St
ABI
lity:
One year from date of receipt when stored as directed.




Application(s):
Great for studying enzyme kinetics and HTS applications.




Reference(s):
Brown MA,
et al. Mol. Cancer
2006;
5:
26.




Notes:

MATERIALS REQUIRED BUT NOT SUPPLIED:

TBST buffer (1x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of re
ADI
ng chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Methyltransferase




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.