商品编号


53013



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Methyltransferase(s)



Size

96 reactions

商品信息













Description:
The SMYD4 Chemiluminescent Assay Kit is designed to measure SMYD4 activity for screening and profiling applications. The SMYD4 Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer, and purified SMYD4 enzyme for 96 enzyme reactions. The key to the SMYD4 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.




Synonym(s):
SET and MYND domain-containing protein 4, KIAA1936




Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt




Format:

COMPONENTS:






Instructions for use:
See product datasheet for detailed protocol.




Storage / St
ABI
lity:
One year from date of receipt when stored as directed.




Application(s):
Great for studying enzyme kinetics and HTS applications.




Reference(s):
Dillon SC, Zhang X, Trievel RC, Cheng X. Genome
BIOLOG
y 2005; 6:227.




Notes:

MATERIALS REQUIRED BUT NOT SUPPLIED:

TBST buffer (1x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of re
ADI
ng chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Methyltransferase




Product Type:
Assay Kit




Data shown is lot-specific.
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for specific information on other lots.