商品编号


60507



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Cell Surface Receptor(s)



Size

2 vials

商品信息





Species: Human

MW: 242 kDa (calc.)





Genbank #: Q15858





Background:
Nav1.7 is a voltage-gated sodium ion channel that in humans is encoded by the SCN9A gene. It is usually expressed at high levels in two types of neurons, the nociceptive neurons at dorsal root ganglion and trigeminal ganglion, and sympathetic ganglion neurons, which are part of the autonomic (involuntary) nervous system. Nav1.7 is present at the endings of pain-sensing nerves, the nociceptors, close to the region where the impulse is initiated.The Nav1.7 channel produces a rapidly activating and inactivating current which is sensitive to the level of tetrodotoxin. Knockout mice that lack Nav1.7 in nociceptors showed reduced response to inflammatory pain.




Description:
Stable recombinant HEK293 cell line expressing human Nav1.7 (Genbank # Q15858) fused at the C-terminus to Green Fluorescent Protein [Ex. ~395 nm, 475 nm; em ~510 nm] and with Cterminal Streptavidin-Binding Peptide (SBP) fusion. Nav1.7 is a tetrodotoxin-sensitive voltagegated sodium channel type IX subunit alpha (SCN9A).




UniProt
Q15858




Synonym(s):
Nav1.7, SCN9A, tetrodotoxin-sensitive voltage-gated sodium channel type IX subunit alpha




Supplied As:
Each vial contains ~2 X 10
6
cells in 1 ml of 10% DMSO.




BIOLOG
ical Activity:
Functionally validated by Western blotting of NaV1.7 using anti-Sodium channel Nav1.7, clone N68/6 (
Millipore
, #MABN41) and Alkaline Phosphatase conjugated anti-mouse IgG. Also validated for NaV1.7 expression by intracellular flow cytometry (FACS) using anti-NaV 1.7 clone 68/6 and PE-labeled anti-mouse IgG.




Host Cell line:
HEK293 cells




Instructions for use:
See cell line data sheet for detailed culturing and assay protocol.




Storage / St
ABI
lity:
Store in liquid nitrogen immediately upon receipt.




Application(s):
?Screen for activators or inhibitors of Nav1.7
?Functional assays for Nav1.7
?Efficient antigen for mouse immunization




Reference(s):
1. Catterall, W.A. Cell and Developmental
BIOLOG
y 16: 521–555 (2000).
2. Choi, J.S., et al. Neurology 67:1563-1567 (2006).
3. Li, Y., el al. Protein Science 20 (1): 140–149 (2011).




Notes:

Contact us for pricing.

License Disclosure
:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suit
ABI
lity of the cell line for any particular use, and does not accept any li
ABI
lity in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bp
SBI
oscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
ion channel




Product Type:
Cell Line




Data shown is lot-specific.
Contact us
for specific information on other lots.