商品编号


62001-1



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Immunotherapy



Size

100 reactions

商品信息













Background:
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells. The L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp. Additionally, the released degradation products of L-Trp have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors and provide cancer cells with an avenue for immune evasion.




Description:
The IDO2 Cellular Activity QuickDetectTM Supplements are designed to complement the hIDO2-HEK293 Recombinant Cell Line (BPS Cat. #60532) or other IDO2-expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of IDO2 and for the analysis and detection of Indoleamine 2,3 dioxygenase 2 (IDO2)-catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.




Synonym(s):
Indoleamine 2,3-dioxygenase 2, IDO2




Format:

Components






Instructions for use:
see data sheet for detailed culturing and assay protocol.




Storage / St
ABI
lity:
Stable for at least 6 months when stored as directed




Application(s):
Monitor IDO2 pathway activity. Screen for activators or inhibitors of IDO2 in a cellular context




Reference(s):
1. Metz, R., et al., Int. Immunol. 2014; 26: 357–367.
2. Fatokun, A., et al., Amino Acids 2013; 45: 1319-1329.




Notes:

Contact us for pricing.

License Disclosure
:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suit
ABI
lity of the cell line for any particular use, and does not accept any li
ABI
lity in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bp
SBI
oscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Immunotherapy




Product Type:
Cell Line




Data shown is lot-specific.
Contact us
for specific information on other lots.