商品编号


90239-B



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Cytokine(s)



Size

10 ?g

商品信息





Species: Mouse

Host Species:
E. coli


MW: 8 kDa





Genbank #: P40224

a.a: 22–93





Background:
SDF-1alpha and SDF-1beta are small cytokines belonging to the CXC- Chemokines. SDF-1 is identical with a chemokine reported to function as a pre-B-cell growth factor in the presence of IL-7 and isolated originally from a murine bone marrow stromal cell line. Human SDF-1alpha and SDF-1beta are encoded by a single gene and arise by alternative splicing. SDF-1 acts on lymphocytes and monocytes but not neutrophils
in vitro
and is a highly potent chemoattractant for mononuclear cells
in vivo
. In addition, SDF-1 also induces intracellular actin polymerization in lymphocytes. SDF acts as a chemoattractant for human hematopoietic
Progen
itor cells expressing CD34 giving rise to mixed types of
Progen
itors, and more primitive types. The chemotactic response is inhibited by pertussis toxin. Chemotaxis of CD34(+) cells in response to SDF is increased by IL-3
in vitro
. SDF has been shown also to induce a transient elevation of cytoplasmic calcium in these cells.




Description:
Recombinant murine stromal cell-derived factor-1beta (SDF1β), also known as CXCL12 or Pre-B cell growth-stimulating factor (PBSF), is a disulfide-linked monomeric protein consisting of 73 a.a. and migrates as an approximately 8 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding mouse Stromal Cell-Derived Factor-1 beta (SDF1β) mature chain was expressed in
E. coli
.




UniProt
P40224




Synonym(s):
stromal cell-derived factor-1β, SDF1β, CXCL12, Pre-B cell growth-stimulating factor, PBSF, SDF1b, SDF-1b




Purity:
≥97% by SDS-PAGE and HPLC




Endotoxin Level:
<0.1 ng/?g (1 EU/?g), using the LAL gel clot method.




BIOLOG
ical Activity:
Determined by its
ABI
lity to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of 5-40 ng/ml.




Formulation:
Lyophilized from a 0.2 ?m filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween-80, 5 mM glutamic acid, pH 4.5.




Format:
lyophilized protein




Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.




Storage / St
ABI
lity:

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.




Reference(s):
1. Shirozu, M., et al. (1995). Genomics 28(3):495-500.
2. Nair, S., Schilling, T.F. (2008). Science 322(5898):89-92.




Warning(s):
Avoid freeze/thaw cycles




Amino Acid Sequence:
KPVSLSYRCPCRFFESHIARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNKRLKM




Scientific Category:
Cytokine/Growth Factors




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.