商品编号


JAI-CCU-400



品牌


Adipogen



公司


Adipogen



公司分类


Assays



Size

100 tests


商品信息


More Information



Product Details



Product Type

Kit



Properties



Application Set

Quantitative
ELISA




Specificity

Specific to Cu
2+
and Cu
+
.



Crossreactivity

All



Quantity

Enough reagents for 100 tests.



Range

3 - 400μg/dL



Sample Type

Cell Lysate
Plasma
Saliva
Serum
Tissue Supernatant
Urine



Detection Type

Colorimetric



Kit Contains

1 x 24 ml ? ?R-1 ? Buffer (with reductant) (ready to use)
1 x 0.5 ml ? R-2 ? Chelate color (ready to use)
1 x 1.2 ml ? STD ?Copper (Cu) standard 200 ?g/dL (ready to use)



Other Product Data

This MC-Reagent Copper Assay Kit is a direct colorimetric assay based on the 3,5-DiBr-PAESA method without deproteinization of the sample. A week acid buffer dissociates copper from the ceruloplasmin-copper complex and is reduced by ascorbic acid (Cu2+ >> Cu+). Cu+ ions form a blue colored complex with the chromogen 3,5-DiBr-PAESA. The color intensity is proportional to the copper concentration in the sample. Absorbance of the Cu
+
-complex is measured at 580nm. Wavelength range of sensitivity: 570 ~ 590nm.

Features of this Assay:
Quick & Easy to use ? Species independent ? Highly sensitive, stable and suitable for high-throughput testing ? No toxic substances ? For cell lysates, serum, plasma and wide variety of
BIOLOG
ical samples



Declaration

Manufactured by JaICA.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

+4°C



Handling Advice

Do not freeze.



Use/St
ABI
lity

12 months after the day of manufacturing. See expiry date on
ELISA
Kit box.



Documents



Manual


Download PDF



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Copper (Cu
+
) is an essential trace element (i.e., micronutrient) and cofactor. 95% of copper in plasma is in complex with enzymes, mostly with ceruloplasmin, several oxidases and dismutases, which play important roles in iron and catecholamine metabolism, free r
ADI
cal scavenging, and in the synthesis of haemoglobin and elastin. The micronutrient is necessary for the proper growth, development, and maintenance of bone, connective tissue, brain, heart, and many other body organs. Copper is involved in the formation of red blood cells, the absorption and utilization of iron, the metabolism of cholesterol and glucose, and the synthesis and release of life-sustaining proteins and enzymes. These enzymes in turn produce cellular energy and regulate nerve transmission, blood clotting, and oxygen transport. Copper stimulates the immune system to fight infections, to repair injured tissues, and to promote healing. Copper also helps to neutralize "free-r
ADI
cals", which can cause severe damage to cells. Copper deficiency lowers the anti-oxidant function in vivo and has been associated with mental retardation, depigmentation, anemia, hypotonia and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as cardiopathy, osteoporosis, osteoarthritis, Menkes syndrome and Wilson's disease. Acute copper toxicity can cause gastroenteritis and acute renal failure.


Product References

Enhanced biofilm formation and/or cell vi
ABI
lity by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor: A. Sakamoto, et al.; Int. J. Biochem. Cell Biol.
44,
1877 (2012)

A Primary Role for Disulfide Formation in the Productive Folding of Prokaryotic Cu,Zn-superoxide dismutase: Y. Sakurai, et al.; J. Biol. Chem.
289,
20139 (2014)