商品编号


JAI-CFE-005



品牌


Adipogen



公司


Adipogen



公司分类


Assays



Size

100 tests


商品信息


More Information



Product Details



Product Type

Kit



Properties



Application Set

Quantitative
ELISA




Specificity

Specific to Fe
2+
and Fe
3+
.



Crossreactivity

All



Quantity

Enough reagents for 100 tests.



Range

5 - 1'000μg/dL



Sample Type

Cell Lysate
Plasma
Saliva
Serum
Tissue Supernatant
Urine



Detection Type

Colorimetric



Kit Contains

1 x 20 ml ? ?R-1 ? Buffer (ready to use)
1 x 0.8 ml ? R-2 ? Chelate color (ready to use)
1 x 4 ml ? ? ?STD ?Iron standard 200 ?g/dL (ready to use)



Other Product Data

This MC-Reagent Iron Assay Kit is a direct colorimetric assay based on the FerroZine? method without deproteinization of the sample. A weak acid buffer dissociates iron from the transferrin-iron complex and is reduced by a reductant (Ferric >> Ferrous). Ferrous ions form a complex with the chromogen Ferrozine. The color intensity is proportional to the iron concentration in the sample. Absorbance of the Fe
2+
-complex is measured at 560nm. Wavelength range of sensitivity: 540 ~ 580nm.

Features of this Assay:
Quick & Easy to use ? Species independent ? Highly sensitive, stable and suitable for high-throughput testing ? No toxic substances ? For cell lysates, serum, plasma and wide variety of
BIOLOG
ical samples



Declaration

Manufactured by JaICA.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

+4°C



Handling Advice

Do not freeze.



Use/St
ABI
lity

12 months after the day of manufacturing. See expiry date on
ELISA
Kit box.



Documents



Manual


Download PDF



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Iron is a mineral (functioning as an enzyme cofactor) that plays an essential role in many
BIOLOG
ical processes. It is essential to nearly all known organisms. As a transition element it can form a range of oxidation states, the most common being Fe
2+
(or ferrous iron) and Fe
3+
(or ferric iron). Ingested iron is mainly absorbed in the form of Fe
2+
. The trivalent form and the heme-bound Fe
2+
-component of iron is reduced by vitamin C. Before passing into the plasma, it is oxidized by ceruloplasmin to Fe
3+
and bound to transferrin to form a transferrin-iron complex. Iron is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Iron-containing proteins participate in many reactions, often utilizing transitory changes in the oxidation state of iron to carry out chemical reactions. Iron is important for redox reactions, oxygen transport (e.g. hemoglobin), short-term oxygen storage (e.g. myoglobin) and energy generation. Iron deficiency has many adverse consequences, including anemia, hemochromatosis, chronic renal disease and in children, behavioral and learning disorders. Iron excess is toxic to the body, harming the heart, liver, skin, pancreatic islet beta cells, bones, joints, and pituitary gland. Maintaining proper iron balance is essential for maintaining homeostasis and health.


Product References

FecA1, a bacterial iron transporter, determines the survival of Helicobacter pylori in the stomach: H. Tsugawa, et al.; Free R
ADI
c. Biol. Med.
52,
1003 (2012)

Estrogen Regulates Hepcidin Expression via GPR30-BMP6-Dependent Signaling in Hepatocytes: Y. Ikeda, et al.; PLoS One
7,
e40465 (2012)

GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance; M. Nakaya; Nat. Comm.
4,
1532 (2013)

Dietary iron restriction inhibits progression of diabetic nephropathy in db/db mice: Y. Ikeda; Am. J. Physiol. Renal. Physiol.
304,
F1028 (2013)

Iron Chelation by Deferoxamine Prevents Renal Interstitial Fibrosis in Mice with Unilateral Ureteral Obstruction: Y. Ikeda; PLoS One
9,
e89355 (2014)