商品编号


SBB-KF0036-KI01



品牌


Adipogen



公司


Adipogen



公司分类


Assays



Size

1 Kit

商品信息


More Information



Product Details



Synonyms

E3 Ubiquitin-protein Ligase Parkin; PARK2; Parkinson Juvenile Disease Protein 2; AR-JP; E3 Ubiquitin Ligase; LPRS2; Parkinson Disease Protein 2; PDJ; PRKN



Product Type

Kit



Properties



Application Set

Compound Screening



Specificity

Readout: Endpoint/Kinetic
Label or Dye: Europium-Cryptate & Cy5
Detection Method: TR-FRET
Substrate Properties: Protein-based Substrate. Typical experimental concentration is 1x.
Excitation/Emission (nm): 304/620 & 304/665



Crossreactivity

Human



Quantity

Sufficient Reagents for:
400 x 20μl reactions
160 x 50μl reactions



Kit Contains

100x UBA1 (100μl)
100x UBE2D3 (100μl)
100x wt-Parkin (100μl)
100x Parkin W304A (100μl)
100x TRF-Ub (phospho pS65) Mix (100μl)
10x Reaction Buffer (1ml)
10x Mg-ATP (1ml)
1 x 384-well white low-volume microplate



Other Product Data

The kit uses ubiquitin labeled with either Europium-Cryptate or Cy5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto Parkin leads to an increase in fluorescence emission at 665nm (Em-Acceptor) and decrease at emission wavelength 620nm (Em-Donor).
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.



Declaration

Manufactured by South Bay Bio.



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.



Documents



Manual

No



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





South Bay Bio’s Parkin TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto both wild-type Parkin and a more active mutant W403A in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto Parkin in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.


Product References

Real-Time Homogenous TR-FRET Ubiquitin Conjugation and Deconjugation Assay Platform: I. Tomasic: Poster, EMBO Conference Ubiquitin and SUMO, Cavtat-Dubrovnik (2017)