商品编号


AG-44T-0137-KI01



品牌


Adipogen



公司


Adipogen



公司分类


Assays



Size

1 Set


商品信息


More Information



Product Details



Synonyms

Small Ubiquitin-related Modifier 1; GAP-modifying Protein 1; SMT3C, SMT3H3, UBL1; Sentrin; Ubiquitin-homology Domain Protein PIC1



Product Type

Set



Properties



Application Set

Compound Screening



Crossreactivity

Human



Kit Contains

50μg of SUMO1 wildtype
50μg of SUMO1 K7R
50μg of SUMO1 K16R
50μg of SUMO1 K17R
50μg of SUMO1 K7R K16R
50μg of SUMO1 K7R K17R
50μg of SUMO1 K16R K17R
50μg of SUMO1 K7R K16R K17R



Other Product Data


Use:
The proteins in this kit have these lysine residues mutated to arginine and can be used to investigate mono-SUMOylation requirements or to reduce poly-SUMO chain formation. Included is wild-type SUMO-1 to be used as a positive control in SUMO-1 conjugation assays which can be performed using the SUMO-1 Conjugation Kit. Typical concentration to support conjugation reaction
in vitro
is 10?M-50?M depending on conditions.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



Manual


Download PDF



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





The ubiquitin-like SUMO-1 is conjugated to a variety of proteins in the presence of Ubc9 and the SUMO E1 activating enzyme (SAE1/SAE2 in human, or Aos1/Uba2p in yeast). The heterodimeric SAE1/SAE2 complex (38 and 70 kDa respectively) uses ATP to adenylate the C-terminal glycine residue of SUMO-1, forming a high-energy thiolester bond with the SAE2 subunit. The second step is the trans-esterification reaction whereby the activated SUMO-1 is transferred to Cys93 of Ubc9. Ubc9 is a member of the E2 family and is homologous to ubiquitin conjugating enzymes, but is specific for the conjugation of SUMO to a variety of target proteins. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation and may play a role in substrate recognition. Sumoylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation, apoptosis, protein function and st
ABI
lity.