商品编号


AG-44T-0135-KI01



品牌


Adipogen



公司


Adipogen



公司分类


Assays



Size

1 Set


商品信息


More Information



Product Details



Synonyms

Small Ubiquitin-like Modifiers



Product Type

Set



Properties



Application Set

Compound Screening



Crossreactivity

Human



Kit Contains

100μg of His-SUMO1
100μg of His-SUMO2
100μg of His-SUMO3



Other Product Data


Use:
Typical concentration to support conjugation reaction
in vitro
is 10?M-50?M depending on conditions.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



Manual


Download PDF



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





The ubiquitin-like SUMO-1, SUMO-2 and SUMO-3 proteins are conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. Human SUMO-1 shares 46% and 47% identity with SUMO-2 and SUMO-3 respectively. SUMO-1 is usually conjugated to proteins as a monomer. SUMO-2 shares 86% identity with SUMO-3, and these isoforms are known to form SUMO chains. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4, E2-25K). Unlike ubiquitination, SUMOylation does not appear to target proteins for degradation by the proteasome. SUMO modification has been implicated in diverse functions such as nuclear transport, chromosome segregation and transcriptional regulation, apoptosis and protein st
ABI
lity.