Adipogen/SUMO1/2/3 (human) (rec.) Protein Set/AG-44T-0136-KI01/1 Set
其他试剂盒
商品编号
AG-44T-0136-KI01
品牌
Adipogen
公司
Adipogen
公司分类
Assays
Size
1 Set
商品信息
More Information
Product Details
Synonyms
Small Ubiquitin-like Modifiers
Product Type
Set
Properties
Application Set
Compound Screening
Crossreactivity
Human
Kit Contains
100μg of SUMO1
100μg of SUMO2
100μg of SUMO3
Other Product Data
Use: Typical concentration to support conjugation reaction
in vitro
is 10?M-50?M depending on conditions.
Declaration
Manufactured by Boston Biochem
Shipping and Handling
Shipping
DRY ICE
Short Term Storage
-20°C
Long Term Storage
-80°C
Handling Advice
Aliquot to avoid freeze/thaw cycles.
Use/St
ABI
lity
Stable for at least 1 year after receipt when stored at -80°C.
Documents
Manual
Download PDF
MSD
S
No
Product Specification Sheet
Datasheet
Download PDF
The ubiquitin-like SUMO-1, SUMO-2 and SUMO-3 proteins are conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. Human SUMO-1 shares 46% and 47% identity with SUMO-2 and SUMO-3 respectively. SUMO-1 is usually conjugated to proteins as a monomer. SUMO-2 shares 86% identity with SUMO-3 and these isoforms are known to form SUMO chains. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4, E2-25K). Unlike ubiquitination, SUMOylation does not appear to target proteins for degradation by the proteasome. SUMO modification has been implicated in diverse functions such as nuclear transport, chromosome segregation and transcriptional regulation, apoptosis and protein st
ABI
lity.
产品货号:2498.4