商品编号


JAI-KPA-050



品牌


Adipogen



公司


Adipogen



公司分类


Assays



Size

1 Kit


商品信息


More Information



Product Details



Product Type

Kit



Properties



Application Set

Quantitative
ELISA




Specificity

In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu++ to Cu+), antioxidant capacity of samples can be detected in 5 minutes. Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine), and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed. PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods and beverage samples.



Crossreactivity

All
Human



Quantity

96 wells



Range

21.9 - 4378 μmol/L (cupric ion reducing power)



Sample Type

Plasma
Serum



Detection Type

Colorimetric



Declaration

Manufactured by JaICA.



Shipping and Handling



Shipping

AMBIENT



Short Term Storage

+20°C



Long Term Storage

+20°C



Handling Advice

Do not freeze.



Documents



Manual


Download PDF



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.


Product References

Restored Antioxidant Capacity Parallels the Immunologic and Virologic Improvement in Children with Perinatal Human Immunodeficiency Virus Infection Receiving Highly Active Antiretroviral Therapy: M. Martino, et al.; Clin. Immunol.
100,
82 (2001)

Effect of epoetin on HO-1 mRNA level and plasma antioxidants in hemodialysis patients: L.A. Calo, et al.; Int. J. Clin. Ther.
41,
187 (2003)

Oxidative stress-related factors in Bartter's and Gitelman9s syndrome: relevance for angiotensin IIsignalling: L.A. Calo, et al.; Nephrol. Dial. Transplant.
18,
1518 (2003)

Vitamin E-coated dialyzers reduce oxidative stress related proteins and
Marker
s in hemodialysis ? a molecular
BIOLOG
ical approach: L.A. Calo, et al.; Clin. Nephrol.
62,
355 (2004)

Oxidative stress and its association with coronary artery disease and different atherogenic risk factors: C. Vassalle, et al.; J. Int. Med.
256,
308 (2004)

Oxidative imbalance and cathepsin D changes as peripheral blood bio
Marker
s of Alzheimer disease: A pilot study: E. Strafacea, et al.; FEBS Lett.
579,
2759 (2005)

Antioxidant capacity as a reliable
Marker
of stress in dairy calves transported by road: P. Pregel, et al.; Vet. Rec.
156,
53 (2005)