商品编号


AG-44T-0139-KI01



品牌


Adipogen



公司


Adipogen



公司分类


Assays



Size

1 Set


商品信息


More Information



Product Details



Product Type

Set



Properties



Application Set

Compound Screening



Kit Contains

25?l of 10X Otubain 1 (OTUB1)
25?l of 10X GST-AMSH
25?l of 10X OTUD3 (catalytic domain)
25?l of 10X His6-Tr
ABI
d
25?l of 10X Cezanne (catalytic domain)
25?l of 10X Yod1
25?l of 10X Otulin (full-length)
25?l of 10X
USP
2 (catalytic domain)
1.5ml of 10X DUB Reaction Buffer
NOTE: Kit contains reagents sufficient for 5 UbiCREST analysis experiments.



Other Product Data


Use:
UbiCREST (Ubiquitin Chain Restriction Analysis) is designed to analyze the ubiquitin linkages present in free polyubiquitin chains or polyubiquitin chains conjugated to a substrate protein. The UbiCREST approach utilizes the Linkage-specificity of the ovarian tumor domain (OTU) class of deubiquitinases (DUBs) to characterize the linkages present in a sample containing polyubiquitin. This kit provides DUBs that are useful for
in vitro
analysis of user supplied polyubiquitinated protein samples. The samples may be generated by
in vitro
ubiquitination reactions, or obtained from cell lysates or other complex mixtures following immunoprecipitation or other means of purification.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



Manual


Download PDF



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Covalent attachment of ubiquitin to target proteins is one of the most versatile and intriguing post-translational modification observed in eukaryotic organisms. Because polyubiquitin chains may be generated by connecting adjacent ubiquitin molecules in one of eight different orientations, a large number of varied architectures may be generated. Polyubiquitination of proteins directs them to different cellular fates such as translocation to different areas within the cell, assembly into multi-subunit complexes, or degradation via the proteasome, depending on the type of linkage used in the polyubiquitin chain. Recent studies have begun to define the roles of these different polyubiquitin signals in physiology and disease, and it is obvious that identifying and understanding the uses of the various linkages will be important in understanding and treating human disease. Polyubiquitin chains are typically formed via the E1, E2, E3 enzyme conjugation cascade where the C-terminal glycine of one ubiquitin is linked to the ε-amino group of a lysine on another ubiquitin; this linkage is referred to as an isopeptide bond. Polyubiquitin chains of all poss
IBL
e linkages including K6-, K11-, K27-, K29-, K33-, K48-, K63-, and Met1-linked (or "linear") have been detected
in vivo
.