商品编号


JAI-CBC-800



品牌


Adipogen



公司


Adipogen



公司分类


Assays



Size

100 tests



商品信息


More Information



Product Details



Synonyms

Unsaturated Iron Binding Capacity Assay Kit



Product Type

Kit



Properties



Application Set

Quantitative
ELISA




Specificity

Detection of unsaturated iron binding capacity (UIBC) by measuring a proportional difference between Fe
2+
and Fe
3+
.



Crossreactivity

All



Quantity

Enough reagents for 100 tests.



Range

10 - 800μg/dL



Sample Type

Plasma
Serum



Detection Type

Colorimetric



Kit Contains

1 x 20 ml ?R-1 ? Buffer (Iron concentration 80μg/dL)?(ready to use)
1 x ? 3 ml ?R-2 ? Chelate color (ready to use)



Other Product Data

This MC-Reagent UIBC Assay Kit is intended for the quantitative determination of unsaturated iron binding capacity (UIBC) in samples by 96-well reader use. The kit utilizes the chromogen bathophenanthroline to bind Fe
2+
. Fe
3+
is then reduced to Fe
2+
by a reducing agent. The Fe
2+
reacts with bathophenanthroline to form a colored complex which is measured by 96-well reader or spectrophotometer and is proportional to the amount of iron present. Absorbance of the Fe
2+
-complex is measured at 546nm and 600nm (reference wavelength).
EDTA-Plasma samples cannot be used due to interference with the chromogenic system.



Declaration

Manufactured by JaICA.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

+4°C



Handling Advice

Do not freeze.



Use/St
ABI
lity

12 months after the day of manufacturing. See expiry date on
ELISA
Kit box.



Documents



Manual


Download PDF



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





Iron is an essential element in mammalians and is contained in various enzymes and is involved in oxidation reactions. Iron is essential during transport of oxygen as composition element of hemoglobin or myoglobin. 30% of the transferrin is associated with Fe
3+
in blood. Free transferrin is not associated to iron. TIBC (Total iron binding capacity) = UIBC (Unsaturated iron binding capacity) + Serum iron. TIBC levels change in blood disorders, hepatic diseases, tumors and inflammation. UIBC levels are increased in patients with iron deficiency. Decreased levels of UIBC are seen in patients with infectious diseases, nephrotic syndrome and low proteinosis.