Adipogen/Zinc Colorimetric Assay Kit (5-Br-PAPS Method)/JAI-CZN-001/100 tests
其他试剂盒
商品编号
JAI-CZN-001
品牌
Adipogen
公司
Adipogen
公司分类
Assays
Size
100 tests
商品信息
More Information
Product Details
Product Type
Kit
Properties
Application Set
Quantitative
ELISA
Specificity
Specific to Zn
2+
.
Crossreactivity
All
Quantity
Enough reagents for 100 tests.
Range
4 - 1000μg/dL
Sample Type
Cell Lysate
Plasma
Saliva
Serum
Tissue Supernatant
Urine
Detection Type
Colorimetric
Kit Contains
1 x 23 ml ? ?R-1 ? Buffer (ready to use)
1 x 0.5 ml ? R-2 ? Chelate color (ready to use)
1 x 1.2 ml ? STD ?Zinc (Zn) standard 200 ?g/dL (ready to use)
Other Product Data
This MC-Reagent Zinc Assay Kit is a direct colorimetric assay based on the 5-Br-PAPS method without deproteinization of the sample. The zinc determination is based on the reaction of zinc with 5-Br-PAPS at alkaline pH in a buffered media, which forms a stable colored complex. The color intensity is proportional to the zinc concentration in the sample. Absorbance of the Zn
2+
-complex is measured at 560nm. Wavelength range of sensitivity: 550 ~ 580nm.
Features of this Assay:
Quick & Easy to use ? Species independent ? Highly sensitive, stable and suitable for high-throughput testing ? No toxic substances ? For cell lysates, serum, plasma and wide variety of
BIOLOG
ical samples
Declaration
Manufactured by JaICA.
Shipping and Handling
Shipping
BLUE ICE
Short Term Storage
+4°C
Long Term Storage
+4°C
Handling Advice
Do not freeze.
Use/St
ABI
lity
12 months after the day of manufacturing. See expiry date on
ELISA
Kit box.
Documents
Manual
Download PDF
MSD
S
No
Product Specification Sheet
Datasheet
Download PDF
Zinc (Zn
2+
) is an essential trace element, vital for the health of animals, plants, and microorganisms . Zinc is found in hundreds of enzymes, is stored and transferred in metallothioneins, and serves as structural ions in transcription factors. Zinc is often coordinated to the sides chains of amino acids, such as aspartic acid, glutamic acid, histidine and cysteine and is involved in nucleid acid and protein synthesis. It is therefore a necessary complement for cell replication. In humans, zinc interacts with a variety of organic ligands, and has roles in nucleic acid metabolism, apoptosis, neurological development, signal transduction, and gene expression. Zinc deficiency is related to skin lesions, irrit
ABI
lity, loss of hair, growth retardation and impaired immunological functions.
Product References
The therapeutic effect on bone mineral formation from biomimetic zinc containing tricalcium phosphate (ZnTCP) in zinc-deficient osteoporotic mice: J. Chou, et al.; PLoS One
8,
e71821 (2013)
A Primary Role for Disulfide Formation in the Productive Folding of Prokaryotic Cu,Zn-superoxide dismutase: Y. Sakurai, et al.; J. Biol. Chem.
289,
20139 (2014)
产品货号:5511.2