商品编号


G1111



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Fluorescent Peptide / Protein Substrates



Size

10 mg

商品信息

(Suc-LLVY)2-Rhodamine is a fluorogenic substrate for the chymotrypsin – like activity of the 20S and 26S proteasomes. Working concentrations of this substrate is 20-100 ?M. The released Rhodamine 110 fluorescence can be detected by a fluorimeter or plate reader at excitation/emission of 495 nm/520nm, respectively. Compared to AMC, Rhodamine 110 is excited at longer wavelength that can significantly enhance the signal/noise ratio. This substrate is extremly valuable for screening small chemical inhibitors of proteasomes by avoiding the interference from chemical compound autofluorescence that often occurs at shorter wavelengths around UV or near-UV regions.

When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Re
ADI
ngs from proteasome inhibitor-treated lysates should be subtracted.

Additional Information






Product Name:

(Suc-Leu-Leu-Val-Tyr)2-Rhodamine 110



Also Known As:

(Suc-LLVY)2-Rhodamine 110



Catalog No.:

G1111



Size:

10 mg



Molecular Weight:

1507.7 Da



Species:

N/A



Source:

Synthetic



Stock:

Powder



Concentration:

N/A



Quality Assurance:

>98% by HPLC and NMR



Storage:

Elig
IBL
e for room temperature shipping. Store at -80°C upon receiving; avoid multiple freeze-thaw cycles after dissolving in DMSO.



PDF Data Sheet:

Download PDF datasheet
,
MSD
S



NCBI RefSeq:

N/A



Image(s):

No



Shipping Method:

Room temperature shipping



References:

No




Details

(Suc-LLVY)2-Rhodamine is a fluorogenic substrate for the chymotrypsin – like activity of the 20S and 26S proteasomes. The working concentration of this substrate is 20-100 ?M. The released Rhodamine 110 fluorescence can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 495 nm/520nm, respectively. Compared to AMC, rhodamine 110 is excited at longer wavelength that can significantly enhance the signal/noise ratio. This substrate is extremly valuable for screening small chemical inhibitors of proteasomes by avoiding the interference from chemical compound autofluorescence that often occurs at shorter wavelengths around UV or near-UV regions. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin should be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Re
ADI
ngs from proteasome inhibitor-treated lysates can be subtracted as background.