商品编号


M3021



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Fluorescent Peptide / Protein Substrates



Size

5x50?g






商品信息

Ub-Rhodamine 110 is a quenched, fluorescent substrate for deubiquitinating enzymes. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine110 results in an increase in rhodamine fluorescence at 535 nm (Exc. 485 nm).


Additional Information






Product Name:

Ub-Rhodamine 110



Also Known As:

N/A



Catalog No.:

M3021



Size:

5x50?g



Molecular Weight:

8936 Da by MS



Species:

Human



Source:

Synthetic



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

N/A



Quality Assurance:

≥95% by RP-HPLC and SDS-PAGE



Storage:

Powder at ?20°C; solution at ?80°C. Avoid multiple freeze/thaw cycles.



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

N/A



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: Ub-Rhodamine 110









Activity assay of Ub-Rhodamine 110 (100 nM) with varying concentrations UCH-L3 (5 - 313 pM).








Reaction buffer: 20 mM Tris, pH 7.1 at 37°C, 150 mM NaCl, 2 mM DTT. Reactions contained 0.5 μM Ub-Rhodamine 110 alone (control, brown line), or with 10 nM GST-
USP
15 (gray line) or with 20 nM GST-
USP
15 (yellow line). Rhodamine110 fluorescence was monitored by using a plate reader with excitation/emission filters at 485/20 and 530/30 nm, respectively.






Shipping Method:

Dry ice shipping



References:

1. A. Tirat
et al
., (2005) Anal. Biochem., 343, 244-255.
2. U. Hassiepin
et al
., (2007) Anal. Biochem., 371, 201-207.




Details

Ub-Rhodamine 110 is a quenched, fluorescent substrate for deubiquitinating enzymes. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine110 results in an increase in rhodamine fluorescence at 535 nm (Exc. 485 nm).

Sample Preparation:
Dilute stock solution into required buffer. Typical reaction concentrations vary from 0.1 - 1 ?M
Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: Ub-Rhodamine 110









Activity assay of Ub-Rhodamine 110 (100 nM) with varying concentrations UCH-L3 (5 - 313 pM).








Reaction buffer: 20 mM Tris, pH 7.1 at 37°C, 150 mM NaCl, 2 mM DTT. Reactions contained 0.5 μM Ub-Rhodamine 110 alone (control, brown line), or with 10 nM GST-
USP
15 (gray line) or with 20 nM GST-
USP
15 (yellow line). Rhodamine110 fluorescence was monitored by using a plate reader with excitation/emission filters at 485/20 and 530/30 nm, respectively.