商品编号


AG-25B-0034-C100



品牌


Adipogen



公司


Adipogen



公司分类


Antibodies



Size

100 ?g


商品信息


More Information



Product Details



Product Type

Polyclonal Antibody



Properties



Source/Host

Rabbit



Immunogen/Antigen

Synthetic peptide corresponding to D
431
EQGEFE(G-COOH*)EEEG
441
-NH2 of human Tubulin β-2A chain (*secondary glycine branched from γ-carboxyl group of glutamate as isopeptide bond).



Application


Western Blot:
(1:10'000)

Immunocytochemistry:
(1:5'000)

Immunoprecipitation:
(1:200)





Crossreactivity

Dog
Human
Mouse



Specificity

This antibody recognizes mono or bi-glycylated Tubulins. The activity of glycylating enzymes (TTLL3 and TTLL8) in cultured cells leads mainly to the modification of α- and β-tubulin, but also of other, yet unidentified protein substrates also detected by the antibody Gly-pep1. In immunofluorescence labeling, the antibody strongly labels glycylated microtubules (Gadadhar, et al.; 2017).



Purity

≥95% (SDS-PAGE)



Purity Detail

Epitope-affinity purified.



Concentration

1mg/ml



Formulation

Liquid. In PBS containing 0.02% sodium azide.



Accession Number

Q13885



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


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Microtubules are key cytoskeletal elements that are found in all eukaryotic cells. Microtubules fulfil a large range of different functions, which are thought to be controlled by the ‘tubulin code’ – mechanism to generate distinct microtubule identities. One mechanism to label specific microtubules are tubulin posttranslational modifications (PTMs), of which a large variety exists. One of these modifications is glycylation, which is generated by the addition of secondary (branched) glycine chains to the main (primary) peptide chain of the protein. The length of these branch chains can vary from one to more than 20 glycine residues. Glycylation is catalysed by the enzymes TTLL3, TTLL8 and TTLL10 from the tubulin tyrosine ligase-like (TTLL) family. Especially TTLL3 and TTLL8 are essential for the initiation of the glycylation because the generate the nascent glycine chain.

The Gly-pep1 antibody was raised against a peptide mimicking beta2-tubulin (TUBB2A) with a single glycine branch on E437. The antibody specifically detects glycylated tubulin, and also other yet unknown glycylation substrates in cells as well as in tissues. As glycyation of microtubules is particularly found in cilia and flagella, Gly-pep1 labels motile cilia as well as primary cilia (Gadadhar et al., 2017)



Product References
Tubulin glycylation controls primary cilia length: S. Gadadhar, et al.; J. Cell Biol.
(Article in press)
(2017)