商品编号


YIF-LF-MA0264



品牌


Adipogen



公司


Adipogen



公司分类


Antibodies



Size

100 ?l

商品信息


More Information



Product Details



Synonyms

VIM; Vimentin



Product Type

Monoclonal Antibody



Properties



Clone

33C7



Isotype

Mouse IgG1 κ



Immunogen/Antigen

Recombinant human His-Vimentin protein purified from E. coli.



Application


ELISA
Western Blot (1:2,000)
Immunoprecipitation (0.5 μl)



Crossreactivity

Human
Rat



Purity Detail

Ammonium sulfate precipitation.



Formulation

Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.



Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.



Declaration

Manufactured by AbFrontier



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Vimentin is a member of the intermediate filament family of proteins found in various non-epithelial cells, especially mesenchymal cells. Vimentin is respons
IBL
e for maintaining cell shape, integrity of the cytoplasm, and st
ABI
lizing cytoskeletal interactions. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Although most intermediate filaments are stable structures, vimentin also has a dynamic nature which is important when offering flexibility to the cell. Two monomers which have central α-helical domains, capped on each end by non-helical domains twist around each other to form a coiled-coil dimer. Two dimers then form a tetramer, which, in turn, form a sheet by interacting with other tetramers. There are some reports related to the biochemical function of intermediate filament network. The intracellular movement of LDL-derived cholesterol from the lysosome to the site of esterification is a vimentin-dependent process. A role for vimentin in mechanotransduction of shear stress has also been suggested. The mechanical stress of fluid shear on endothelial cells seems to trigger MAPK signaling pathways and stimulates proliferation. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.


Product References
1) Pekny M and Lane EB, (2007) Exp Cell Res. 313(10):2244-2254 (General)
2) Holwell TA et al., (1999) J Lipid Res. 40(8):1440-1452. (General)
3) Henrion D et al., (1997) J. Clin. Invest. 100:2909–2914 (General)
4) Sarria AJ et al., (1992) J Biol Chem. 267(27):19455-19463. (General)