商品编号


YIF-LF-MA0197



品牌


Adipogen



公司


Adipogen



公司分类


Antibodies



Size

100 ?l

商品信息


More Information



Product Details



Synonyms

SRK; ZAP70; EC=2.7.10.2; Syk-related Tyrosine Kinase; Tyrosine-Protein Kinase ZAP-70; 70 kDa zeta-Chain Associated Protein



Product Type

Monoclonal Antibody



Properties



Clone

49B4



Isotype

Mouse IgG1 κ



Immunogen/Antigen

Recombinant human A
BD
-ZAP70 protein purified from E. coli.



Application


ELISA
Western Blot (1:10,000)
Immunoprecipitation (2 μl)



Crossreactivity

Human



Purity Detail

Ammonium sulfate precipitation.



Formulation

Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.



Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.



Declaration

Manufactured by AbFrontier



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





ζ-chain associated protein kinase, ZAP70, is a 70 kDa member of the Syk family kinase predominantly involved in T cell receptor (TCR) signaling. It is structurally homologous to Syk, a PTK that is involved in proximal BCR signaling. ZAP-70 is a key signaling molecule in T cell activation and also plays a role in apoptosis and cell migration. SYK family tyrosine kinases contain a C-terminal kinase domain and tandem N-terminal SH2 domains that bind phosphorylated ITAMs (immunoreceptor tyrosine-based activation motif). Linker region that contains multiple tyrosines separates the SH2 domains from the kinase domain. Phosphorylated tyrosines act as docking sites for phospholipase Cγ1 (PLCγ1). ZAP-70 and Syk are functionally homologous in antigen receptor signaling. Expression of ZAP-70 in Syk? B cells reconstitutes SCR function. Reconstitution requires the presence of functional Src homology 2 (SH2) and catalytic domains of ZAP-70. Expression of ZAP-70 is an important negative prognostic factor in chronic lymphocytic leukemia (CLL) with more rapid disease progression and shorter survival. Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.


Product References
1) Gobessi S. et al., (2007) Blood. 109:2032-2039. (General)
2) Orchard J.A. et al., (2004) Lancet. 363:105-111. (General)
3) Kong G.H. et al., (1995) Immunity. 2:485-492. (General)