商品编号


P1010-100



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Protein Expression / Purification Reagents



Size

100 g


商品信息

IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in
E. coli
. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate.

Note: To prepare 10 mL 1M IPTG stock solution, weight 2.38 g IPTG and dissolve in 8 ml distilled water, adjust to a final volume of 10 ml with distilled water. Sterile the solution using a 0.22 um syringe filter. Aliquot the stock and store under -20°C for up to one year.

Additional Information






Product Name:

IPTG



Also Known As:

isopropyl - β - D - thiogalactopyranoside



Catalog No.:

P1010-100



Size:

100 g



CAS:

367-93-1



Formula:

C
9
H
18
O
5
S



Molecular Weight:

238.3



Form:

Powder



Quality Assurance:

>99% by HPLC, dioxane-free



Source:

Synthetic



Storage:

Elig
IBL
e for ambient temperature shipping. Store at 4°C upon receiving.



PDF Data Sheet:

PDF Datasheet



Image(s):




Shipping Method:

Room temperature shipping



References:

J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, A1.27, 2001) pp. 1.124-1.125.




Details

IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in
E. coli
. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate.
Note: To prepare 10 mL 1M IPTG stock solution, weight 2.38 g IPTG and dissolve in 8 ml distilled water, adjust to a final volume of 10 ml with distilled water. Sterile the solution using a 0.22 um syringe filter. Aliquot the stock and store under -20°C for up to one year.

Expression of GST-UCHL3 in BL21 (DE3) cells was induced by the addition of 25 μM (lane 3), 100 μM (lane 4), 200 μM (lane 5) or 400 μM (lane 6) IPTG at 37 0C for 2 hours. Lane 2 without IPTG.