商品编号


P1011-10



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Protein Expression / Purification Reagents



Size

10 ml


商品信息

IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in
E. coli
. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate.


Additional Information






Product Name:

IPTG Solution (1M)



Also Known As:

isopropyl - β - D - thiogalactopyranoside



Catalog No.:

P1011-10



Size:

10 ml



CAS:

367-93-1



Formula:

C
9
H
18
O
5
S



Molecular Weight:

238.3



Form:

Liquid. Sterilized by filtration through a 0.22 micron filter.



Quality Assurance:

>99% by HPLC, dioxane-free



Source:

Synthetic



Storage:

Elig
IBL
e for ambient temperature shipping. Store at 4°C upon receiving.



PDF Data Sheet:

PDF Datasheet



Image(s):




Shipping Method:

Wet ice shipping



References:

J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, A1.27, 2001) pp. 1.124-1.125.




Details

IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in
E. coli
. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate.

Expression of GST-UCHL3 in BL21 (DE3) cells was induced by the addition of 25 μM (lane 3), 100 μM (lane 4), 200 μM (lane 5) or 400 μM (lane 6) IPTG at 37 0C for 2 hours. Lane 2 without IPTG.