商品编号


016-050-084



品牌


Jackson



公司


Jackson ImmunoResearch Laboratories



公司分类


Streptavidin and Conjugates



Conjugate

Alkaline Phosphatase


Unit

1.0 mg


Product

Streptavidin


产地

美国

商品信息

Steptavidin, a bacterial protein isolated from
Streptomyces avidinii
, is similar to egg-white avidin in its
ABI
lity to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.
Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.
Physical State:
Sterile-filtered liquid
Storage:

Store at 2-8°C. Prepare working dilution fresh each day. For extended storage, add an equal volume of glycerol (ACSgrade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: adding glycerol reduces the stated protein concentration and dilution range by one-half.
Expiration date:
one year from the date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Buffer:
0.01M Tris-HCl, 0.25M NaCl, pH 8.0
St
ABI
lizer:
15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative:
0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
ELISA
:- 1-2 ?g / ml
Western Blot:- 0.1-1 ?g / ml
Histo-/Cyto-Chemistry:- 1-2 ?g / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, perme
ABI
lity, etc. The actual dilution used must be determined empirically.
Alkaline Phosphatase
Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas
et al
., Scand. J. Immunol. 1978.
8
(Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as
ELISA
and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.