商品编号


GWB-429DDB



品牌


GenWay



公司


GenWay



公司分类


Secondary Antibodies



Size

1 mg

商品信息

Additional Information:




Name

Mouse IgG Antibody (Biotin)



Related Product Names

RABBIT F(ab')2 ANTI MOUSE IgG: Biotin IgGIGHG1



Gene Symbol

Ighg1



Gene Family

IGH



Gene id

16017



Alias Symbols

IgG1, Igh-4, VH7183, Ighg1



Protein Name

Ig gamma-1 chain C region, membrane-bound form



Description of Target

RABBIT F(ab')2 ANTI MOUSE IgG:Biotin



Swissprot ID

P01869



Species Reactivity

Mouse



Host

Rabbit



Isotype

Polyclonal IgG



Conjugation

Biotin



Replacement

This antibody may replace item sc-52003 from Santa Cruz Biotechnology.



Immunogen

Purified mouse IgG



Homology

Mouse



Product Format

F(ab')2 fragment of IgG conjugated to biotin - liquid



Swiss Prot Number

P01869



Specificity

IgG



Concentration

1.0mg/ml



Applications

C, E, F, P



Application Info

Flow Cytometry:
? ?1/50 - 1/100
Immunohistology - Frozen:
? ?1/50 - 1/200
Immunohistology - Paraffin:
? ?1/50 - 1/200
ELISA
:
? ?1/1000 - 1/2000



Clonality

Polyclonal



Format

Solution: Phosphate buffered saline, Stabalizer: 0.09%, Sodium Azide, Form: F(ab')2 fragment of IgG conjugated to biotin - liquid



St
ABI
lity

18 months from date of despatch.



Reconstitution and Storage

Store at +4
o
C or at -20
o
C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.



Reactivity

Mouse



Storage

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate



Protocol Information

Citation:
1: Turner OC, Basaraba RJ, Orme IM. Immunopathogenesis of pulmonary granulomas inthe guinea pig after infection with Mycobacterium tuberculosis. Infect Immun.2003 Feb;71(2):864-71. PubMed PMID: 12540568; PubMed Central PMCID: PMC145351.
Species:
Guinea pigs, M. tuberculosis
Experiment Name:
Immunohistochemistry
Experiment Background:
1. Localization of lymphocyte subsets by immunohistochemistry.2. Pulmonary tuberculosis in guinea pigs is similar to the disease in humans and is accordingly widely used as a model to test tuberculosis vaccines. The primary site of expression of acquired immunity and the hallmark of tuberculosis is the granuloma. Granuloma morphology is well described, but there is limited information regarding T-cell subset influx. Oliver and his team monitored the course of pulmonary tuberculosis in guinea pigs and observed four distinct immunohistopathological stages. In all stages there were similar numbers and arrangement of CD4 and CD8 T cells. There were only small numbers of apoptotic lymphocytes, scattered around and within the necrotic core, and acid-fast bacilli were vis
IBL
e both within macrophages and free within airway debris. A key finding of the study was the observation that the development of the necrotic core was an early event and almost certainly preceded the emergence of the acquired immune response. This in turn suggests that innate mechanisms are the basis of the early lesions and that subsequent acquired responses are unable to moderate them. This hypothesis differs from the current dogma that excessive activity of T cells mediates delayed-type hypersensitivity and that cellular cytolysis is the root cause of the necrosis.
Experimental Steps:
Mouse anti-guinea pig monoclonal antibodies specific for guinea pig CD4 (clone CT7) and CD8 (clone CT6) were purchased from (Oxford, England). In each case, the mouse immunoglobulin G1 (IgG1)-negative control (clone W3/25) was used as the isotype control. F(ab’)2 rabbit anti-mouse Ig-biotin, also from was used as the secondary antibody At necropsy, the left cranial lung lobe was first slowly infused with a 20% OCT (Tissue-Tek, Inc. Torrance, Calif.)–phosphate-buffered saline solution through the major vessels at the hilus. The samples were then placed in a tissue mold, completely surrounded by OCT, frozen in a bath of liquid nitrogen, and stored at -70°C until used. Serial sections from each lung, 5 ?m thick, were cut in a cryotome (CM 1850;
Leica
, Bannockburn, Ill.) by employing the Instrumedics Inc. (Hackensack, N.J.) tape transfer system, fixed in cold acetone for 5 to 10 min, and then air dried. Next, the sections were washed in APK buffer solution (Vetana Medical Systems, Tucson, Ariz.) for 15 to 20 min and incubated in a 1:50 (CD4) or 1:100 (CD8) solution of Protein Block goat serum (Biogenex, San Ramon, Calif.) and primary antibody for 30 min. The sections were then placed on a Nexus automated immunostainer (Ventana Medical Systems). The labeled avidin-alkaline phosphatase and Fast red/napthol detection kit was employed. The secondary antibody was incubated for 30 min at room temperature. Sections were counterstained with Meyer’s hematoxylin. Sections of spleen were also examined to act as positive controls.
Number Of Protocols:
1



Lead Time

Domestic: within 2-3 weeks delivery?International: 2-3 weeks



Intended Use

Research Use Only



Key Reference

1. Silveira, M. et al. (2002) Infection with Strongyloides Venezuelensis induces transient airway eosinophilic inflammation, an increase in immunoglobulin E and hyperresponsiveness in rats. Infect. Immun. 70 (11): 6263 - 6272



::

Preservative St
ABI
lisers:
0.09% - Sodium Azide
Antiserum Preparation:
Antisera to Mouse IgG were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')
2
fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.



::

Approx Protein Conc:
F(ab')2 concentration 1.0mg/ml
Buffer Solutions:
Phosphate buffered saline pH7.2