商品编号


GWB-5AEE17



品牌


GenWay



公司


GenWay



公司分类


Secondary Antibodies



Size

1 ml

商品信息

Additional Information:




Name

Rabbit F(ab')2 Anti-Human IgG (H+L) Absorbed against mouse immunoglobulins



Related Product Names

Rabbit F(ab')2 Anti-Human IgG(H+L), Mouse ads-HRP; N/A Rabbit F(ab')2 Anti-Human IgG (H+L) Absorbed against mouse immunoglobulinsIGHG1



Gene Symbol

IGHG1



Gene Fullname

Ig gamma-1 chain C region



Protein Name

Ig gamma-1 chain C region



Swissprot ID

P01857



Host

Pepsin digest of rabbit anti- human IgG (H+L).



Isotype

IgG



Replacement

This antibody may replace item sc-52003 from Santa Cruz Biotechnology.



Product Format

Horser
ADI
sh Peroxidase (HRP) Conjugate



Swiss Prot Number

P01857



Purification

Affinity chromatography on human IgG covalently coupled to agarose. Gelfiltration chromatography of pepsin digested antibody.



Specificity

Reacts with the heavy and light chains of human IgG as demonstrated by
ELISA
or FLISA. May also react with the light chains of other human immunoglobulins.Minimal cross reactivity with mouse immunoglobulins.



Applications

IF,
ELISA
, WB, DB, IHC



Application Info

- Immunofluorescent staining
- Enzyme-Linked-Immunosorbent-Assay (
ELISA
)
- Fluorescent-Linked-Immunosorbent-Assay (FLISA)
- Western blotting
- Dot-and slot-immunoblotting
- Immunohistochemistry



Dilution

FLISA:
ELISA
:
FITC conjugate
AP conjugate
HRP conjugate
1:200 – 1:400
1:2,000-1:4,000
1:4,000-1:8,000



Clonality

Polyclonal



Format

Horser
ADI
sh Peroxidase (HRP) Conjugate



Absorption

Pooled mouse immunoglobulins.



Reactivity

Human



Warning

Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.



Storage

The horser
ADI
sh peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8C or long-term at -20C.



Protocol

Reconstitution Instructions
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme- Linked ImmunoSorbent Assay (
ELISA
) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol



Lead Time

Domestic: within 1-2 weeks delivery?International: 1-2 weeks



Intended Use

Research Use Only