商品编号


R400585



品牌


Rubicon Genomics



公司


Rubicon Genomics



公司分类


Products



Size

1 Ea





商品信息

.detail-txt img { max-width: 100%; }







Reduce Background for Results You Can Trust

ThruPLEX Tag-seq libraries were prepared from 30 ng of the 1% Horizon cfDNA standard. Following
Agilent
SureSelect target enrichment and sequencing to achieve ~10,000X coverage, data was analyzed with the Curio Genomics bioinformatics platform. Using the raw reads, the EGFR mutation (yellow dot) is obscured by background errors (left). The mutation is re
ADI
ly identified when UMTs are utilized in data processing to correct for errors (right).








Dramatically Increase the Signal-to-Noise Ratio to Discover More

Data from either the 30 ng Horizon cfDNA library (previous slider) (A) or a ThruPLEX Tag-seq library prepared from 10 ng Horizon cfDNA standard, enriched with a custom 240 KB panel (NimbleGen SeqCapEZ) and sequenced on a HiSeq 2500 to achieve ~5,000X coverage. Analysis was performed using the Curio Genomics bioinformatics platform.








Single-Tube, Three Step Workflow Helps your Precious Samples Go Further

Starting with 1 to 50 ng of DNA, ThruPLEX Tag-seq Kit creates indexed libraries in 3 simple steps: end repair, adapter ligation, and high-fidelity library amplification. No purification or sample transfer steps are required. The streamlined workflow is performed in 2 hours in a single tube or well, preventing sample loss and enhancing positive sample identification.








ThruPLEX Technology Generates High Quality libraries the first time, every time

ThruPLEX technology is a 3-step reaction that starts with fragmented double-stranded DNA or cell-free DNA which is repaired in a highly efficient process. Background is reduced using double-stranded adaptors with no single-stranded tails. Blunt end ligation occurs with high-efficiency. Blocked 5' ends reduce adapter-adapter ligation.








Time Saving Bioinformatics Solutions

Reach results quickly with the Curio Genomics cloud-based software (www.curiogenomics.com) or Connor, an open-source tool available for data processing (https://github.com/umich-brcf-bioinf/Connor).




Guaranteed for 9 months at -20 °C in a constant temperature freezer.

Store at –20 °C.


CAT. NO. R400584 ThruPLEX Tag-seq 6S? Kit (12 reactions, 6 single indexes)

CAT. NO. R400585 ThruPLEX Tag-seq 48S Kit (48 reactions, 48 single indexes)

CAT. NO. R400586 ThruPLEX Tag-seq 96D Kit (96 reactions, 96 dual indexes)

ThruPLEX Tag-seq contains all necessary reagents for preparing indexed
Illumina
NGS libraries, including optimized
Illumina
-compat
IBL
e adapters and indexing reagents.

How to Buy


Results you can trust
. ThruPLEX Tag-seq technology provides up to 16 million molecular tags to correct sequencing errors providing confident variant detection.

Discover more, cost effectively
. Use with hybridization-based target enrichment systems to examine hundreds of genes, including mutations and structural variants, simultaneously.

High quality libraries the first time, every

time
. Unparalleled ease of use reduces user error and contamination with our single-tube, 2-hour, 3-step workflow.

Time-saving bioinformatics solutions
. Reach results quickly with cloud-based software from Curio Genomics?or open-source scripts available for data processing.

Precious samples go further
. Use less DNA to analyze samples once too low to detect. ThruPLEX Tag-seq works with input amounts from 1 ng to 50 ng of cfDNA or fragmented dsDNA to accommodate a wide range of samples.