商品编号


R400491



品牌


Rubicon Genomics



公司


Rubicon Genomics



公司分类


Products



Size

1 Ea






商品信息

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Highest Diversity, Fewest Unmapped Reads from Cell-Free DNA

ThruPLEX Plasma-seq Kit generated quality libraries with high diversity and a low number of duplicates and unmapped reads. Cell-free DNA was extracted from 3 plasma samples, and libraries were prepared at the amounts indicated as measured by
qubit
?. The amount of mononucleosomal DNA in each sample as measured by the Bioanalyzer? corresponded to 0.09 ng, 0.62 ng, and 15.44 ng. Pooled libraries were sequenced on an
Illumina
NextSeq? 500 as a paired-end run with 17M to 25M reads per library, Duplication rates were calculated after down-sampling the data to 17 M reads per library.








Outstanding Target Enrichment Peformance

ThruPLEX Plasma-seq Kit libraries were captured at high efficiency and generated data with deep coverage of the kinome for mutation detection. Libraries were prepared from 3 plasma samples at input amounts of 5 ng, 6.5 ng, and 10 ng in triplicate, and targeted sequencing was carried out on an
Illumina
MiSeq using samples enriched with the ClearSeq Human DNA Kinome Panel for SureSelectXT2. On average, 5M reads were generated per library. Selected bases were successfully captured bases that were in or within 250 bp of the baits.








Reproduc
IBL
e, Unbiased GC Coverage

ThruPLEX Plasma-seq Kit provided the most reproduc
IBL
e and unbiased GC coverage across the human genome. ThruPLEX libraries showed minimal vari
ABI
lity across 9 individual plasma samples tested. Libraries were prepared from cell-free DNA isolated from 1 mL of plasma samples and sequenced on an
Illumina
NextSeq 500. Four separate plasma samples were used to construct the
NEB
Next Ultra libraries.








Evaluated at Karolinska Institute and CRUK

Illumina
NGS libraries were prepared with 5 ng of cell-free DNA isolated from pooled plasma samples using ThruPLEX Plasma-seq Kit. Low-pass WGS was conducted on a HiSeq with approximately 12.5M reads per library. (Data courtesy of the Karolinska Institute, Sweden).








ThruPLEX Plasma-seq Kit Single-Tube Workflow

Starting with 1 to 30 ng of cell-free DNA, ThruPLEX Plasma-seq Kit creates indexed libraries in 3 simple steps: end repair, adapter ligation, and high-fidelity library amplification. No purification or sample transfer steps are required. The streamlined workflow is performed in 2 hours in a single tube or well, preventing sample loss and enhancing positive sample identification.








ThruPLEX Plasma-seq Kit Technology

ThruPLEX Plasma-seq Kit technology is a 3-step reaction that is optimized for cell-free DNA. Cell-free DNA (1 ng to 30 ng) is first repaired in a highly efficient process. Background is reduced using double-stranded adapters with no single-stranded tails. Blunt-end ligation occurs with high-efficiency. Blocked 5’ ends reduce adapter-adapter ligation. Background is further reduced by destroying unused adapters after ligation. A high-fidelity amplification completes the reaction to generate indexed
Illumina
libraries.




Guaranteed for 9 months at -20°C in a constant temperature freezer.

Store at –20 °C.


CAT. NO. R400490 ThruPLEX Plasma-seq 12S? Kit (12 reactions, 12 single indexes)

CAT. NO. R400491 ThruPLEX Plasma-seq 48S Kit (48 reactions, 48 single indexes)

CAT. NO. R400492 ThruPLEX Plasma-seq 96D Kit (96 reactions, 96 dual indexes)

ThruPLEX Plasma-seq contains all necessary reagents for preparing indexed
Illumina
NGS libraries, including optimized
Illumina
-compat
IBL
e adapters and indexing reagents.

?


Designed for cell-free DNA
: newly formulated repair and ligation reagents with optimized protocols



High performance NGS libraries
: high diversity with broad and reproduc
IBL
e GC coverage



Variable sample input
: <1 ng to 30 ng of cell-free DNA



Fast and simple workflow
: 3 steps in a single tube or well in 2 hours with no purification or sample transfer steps



Research applications
: libraries from samples where results require high sensitivity including liquid biopsies, ctDNA, and targeted sequencing



Automation-friendly
:
Beckman
?
FX
P
Workstation