商品编号


4676-096-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


DNA Damage



Size

96 tests

商品信息

Description

This
ELISA
based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.

FEATURES:


Chemiluminescent readout

96 strip-well format

Sensitive – detects 10 mU PARP /well

Assay Time ~3 hrs


APPLICATIONS:
For the
in vitro
screening of candidate PARP-1 inhibitors and determination of IC50 values.




Catalog # 4676-096-K includes :



Catalog Number

Description

Qty



4667-50-03

3-Aminobenzamide

1



4668-050-01

Human PARP 1 Enzyme, HSA

1



4671-096-02

20X PARP Buffer

2



4671-096-03

10X PARP Cocktail

1



4671-096-06

10X Activated DNA

1



4671-096-04

10X Strep-Diluent

1



4675-096-01

PeroxyGlow? A

1



4675-096-02

PeroxyGlow? B

1



4678-096-P

Histone-Coated White Strip Well Plate

1



4800-30-06

Strep-HRP

1




Role for DNA Damage Signaling in Pulmonary Arterial Hypertension
Meloche, J., Pflieger, A., Vaillancourt, M., Paulin, R., Potus, F., Zervopoulos, S., Graydon, C., Courboulin, A., Breuils-Bonnet, S., Tremblay, E., Couture, C., Michelakis, E.D.,
Circulation, Feb 2014; 129: 786 – 797
http://circ.ahajournals.org/cgi/content/abstract/129/7/786

YU238259 Is a Novel Inhibitor of Homology-Dependent DNA Repair That Exhibits Synthetic Lethality and R
ADI
osensitization in Repair-Deficient Tumors
Gregory C. Stachelek, Elizabeth Peterson-Roth, Yanfeng Liu, Rafael J. Fernandez, III, Luke R.G. Pike, Jack M. Qian, Laura Abriola, Denton Hoyer, William Hungerford, Janie Merkel, and Peter M. Glazer
Mol. Cancer Res., Oct 2015; 13: 1389 – 1397.
http://mcr.aacrjournals.org/cgi/content/abstract/13/10/1389

“Latonduine Analogs Restore F508del–Cystic Fibrosis Transmembrane
Conductance Regulator Trafficking through the Modulation of Poly-ADP
Ribose Polymerase 3 and Poly-ADP Ribose Polymerase 16 Activity”
“Graeme W. Carlile, Renaud Robert, Elizabeth Matthes, Qi Yang, Roberto Solari, Richard Hatley, Colin M.
Edge, John W. Hanrahan, Raymond Andersen, David Y. Thomas, and Véronique Birault”
Mol. Pharmacol., Jul 2016; 90: 65 – 79.
http://molpharm.aspetjournals.org/cgi/content/abstract/90/2/65