商品编号


7512-100-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


Oxidative Damage



Size

5 x 96 Wells

商品信息

Description

7512-100-k_cuvette_assay_calculation_worksheet

7512-100-k_plate_assay_calculation_worksheet

Tetrameric Glutathione Peroxidase catalyzes the reduction of H2O2 to water, and organic peroxides to the corresponding stable alcohols, by using glutathione as a source of reducing equivalents. It requires selenium as a cofactor and contains a selenocysteine amino acid residue in the active site of each monomer that participates in the actual mechanism of the enzyme. Glutathione Reductase (GR), provided with each kit, then reduces the oxidized glutathione to complete the cycle. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the Glutathione Peroxidase activity in the sample.

FEATURES:


Suitable for a 96 well or cuvette format

Suitable for plasma, erythrocyte lysates, tissue homogenates, and cell lysates


APPLICATIONS:

Calculating glutathione peroxidase activity.

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Catalog # 7512-100-K includes :



Catalog Number

Description

Qty



7512-100-01

Glutathione Peroxidase

1



7512-100-02

10X Assay Buffer

1



7512-100-03

GSH+NADPH

1



7512-100-04

Glutathione Reductase

1



7512-100-05

Cumene Hydroperoxide

1



7512-100-06

96-well plates

1




Glutathione-Mediated Detoxification of Halobenzoquinone Drinking Water Disinfection Byproducts in T24 Cells
Jinhua Li, Wei Wang, Hongquan Zhang, X. Chris Le, and Xing-Fang Li
Toxicol. Sci., Oct 2014; 141: 335 – 343.
http://toxsci.oxfordjournals.org/cgi/content/abstract/141/2/335