商品编号


M0259S-200 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


PCR, qPCR & Amplification Technologies




200 units


Concentration

2,000 units/ml

商品信息

Description:
Deep Vent (exo-) DNA Polymerase has been genetically engineered to eliminate the3?→ 5?proofre
ADI
ng exonuclease activity associated with Deep Vent DNA Polymerase. Deep Vent (exo-) DNA Polymerase is even more stable than Vent
?
(exo-) DNA Polymerase with a half-life of 23 hours at 95°C and 8 hours at 100°C. Both Deep Vent (exo-) and Vent (exo-) DNA Polymerase are suitable for primer extensions and high temperature (72°C) DNA sequencing.
Deep Vent DNA Polymerase crystals (Ira Schildkraut and Rebecca Kucera,
New England Biolabs
, Inc.)
Highlights
Isolated from a recombinant source
Supplied with 10X Reaction Buffer and 100 mM MgSO
4
Extremely High
Thermo
st
ABI
lity: half-life of 23 hours at 95°C
Difficult Templates; ideal for GC-rich or looped sequences
Product Source
An
E. coli
strain that carries the Deep Vent(D141A / E143A) DNA Polymerase gene, a genetically engineered form of the nativeDNA polymerase from
Pyrococcus
species GB-D. The native organism wasisolated from a su
BMA
rine thermal vent at 2,010 meters (1) and is able to growat temperatures as high as 104°C.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
Magnesium Sulfate (MgSO
4
) Solution
-20
100 mM
Thermo
Pol
??
Reaction Buffer Pack
-20
10X
Notes:
BSA is not provided with this enzyme since its presence is not necessary for most primer extension reactions. However, it is available as
NEB
#B9001. Acetylated BSA should not be used for primer extension reactions.Diluent D is also available (#B8004). This diluent is recommended for making dilutions of Deep Vent (exo-) DNA Polymerase.Additional
Thermo
Pol Reaction Buffer Packs are also available (#B9004). Each buffer pack contains 4 vials of 10X buffer (1.5 ml each) and 1 vial of 100 mM MgSO4.