商品编号


R0505S-2,500 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

10,000 units/ml


Size

2,500 units

商品信息

Description:
EagI has a High Fidelity version EagI-HF? (
NEB
#R3505).
High Fidelity (HF?) Restriction Enzymes have 100% activity in CutSmart? Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.HF enzymes also exhibit dramatically reduced star activity.HF enzymes are all Time-Saver? qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA.Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Product Source
An
E. coli
strain that carries the EagI gene from
Enterobacter agglomerans
(R. Morgan).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
NEB
uffer? 3.1
-20
10X
Notes:
EagI is an isoschizomer of XmaIII.For full EagI activity, the pH of the reaction mix must be between (7.9 and 9.0 @ 25°C).This enzyme has shown to have lower activity on some supercoiledplasmids, with more than 1 unit required to digest 1 μg plasmid DNA. Forcomplete digestion of 1 μg of plasmid DNA please follow our recommendeddigestion protocol.