商品编号


R0101S-10,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

20,000 units/ml


Size

10,000 units

商品信息

Description:
EcoRI has a High Fidelity version EcoRI-HF? (
NEB
#R3101).
High Fidelity (HF?) Restriction Enzymes have 100% activity in CutSmart? Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.HF enzymes also exhibit dramatically reduced star activity.HF enzymes are all Time-Saver? qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA.Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
For details on
NEB
’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.
Product Source
An
E. coli
strain that carries the cloned EcoRI gene from
E. coli
RY13 (R.N. Yoshimori).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
NEB
uffer? EcoRI
-20
10X
Notes:
May exhibit star activity in
NEB
uffer 2.1 or CutSmart Buffer.This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol.