商品编号


R0641S-300 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

5,000 units/ml


Size

300 units

商品信息

Description:
Product Source
An?
E. coli
?strain that carries the AcuI gene from?
Acinetobacter calcoaceticus
?(S.K. Degtyarev).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
S-adenosylmethionine (SAM)
-20
32 mM
CutSmart? Buffer
-20
10X
Notes:
Requires S-adenosylmethionine for optimal activity. (supplied with enzyme)Storage of SAM:?S-adenosylmethionine is stored at –20°C as 32?mM solutiondissolved in sulfuric acid (0.005?M) and 10% ethanol. SAM in this solutionstored under ideal conditions remains active for up to 6?months. SAM is unstableat (pH 7.5), 37°C, and should be replenished for reactions incubated longer than4 hours. Many problems in achieving complete digestion can be alleviated byusing fresh SAM.Based on the st
ABI
lity of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.Star activity may result from extended digestion, high enzyme concentration or a glycerol concentration of >5%