商品编号


M0533S-500 reactions (50 μl vol)



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类






500 reactions (50 μl vol)


Concentration

2X

商品信息

Description:
LongAmp Hot Start
Taq
2X Master Mix contains a unique blend of aptamer-based Hot Start
Taq
and Deep Vent
R
? DNA Polymerases. The aptamer-based inhibitor binds revers
IBL
y to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. The LongAmp? Hot Start
Taq
2X Master Mix does not require a separate high temperature incubation step to activate the enzyme. The 3?→5? exonuclease activity of Deep Vent
R
DNA Polymerase increases the fidelity and robust amplification of Hot Start
Taq
DNA Polymerase (1). LongAmp? Hot Start
Taq
DNA Polymerase offers two-fold higher fidelity than Hot Start
Taq
DNA Polymerase alone. The convenient master mix formulation is supplied at a 2X concentration and contains dNTPs and Mg++, requiring only the addition of primers and DNA template for robust amplification. A wide range of PCR products can be generated; up to 30 kb from lam
BD
a or human genomic DNA.
Amplification of a 8 kb amplicon from varying amounts of Jurkat genomic DNA using LongAmp Hot Start Taq 2X Master Mix. Starting template amounts are indicated below the gel.
Marker
M is the
NEB
2-Log DNA Ladder (
NEB
#N3200 ).
Product Source
An
E. coli
strain that carries the
Taq
DNA Polymerase gene from
Thermus aquaticus
YT-1 and an
E. coli
strain that carries the Deep Vent
R
DNA Polymerase gene from
Pyrococcus
species GB-D.
Unit Definition:
Oneunit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPinto acid insoluble material in 30 minutes at 75°C.
Unit AssayConditions:
1X
Thermo
Pol? Reaction Buffer, 200 ?M dNTPs including[
3
H]-dTTP and 200??g/ml activated Calf Thymus DNA.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
LongAmp
?
?
Taq
Reaction Buffer Pack
-20
5X
Notes:
Product specifications for individual components in the LongAmp Hot Start Taq?2X Master Mix are available separately.