商品编号


R3160S-3,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类





Concentration

20,000 units/ml


Size

3,000 units

商品信息

Description:
Product Source
An
E. coli
strain that carries the cloned and modified BclI gene from
Bacillus caldolyticus
(A. Atkinson).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Gel Lo
ADI
ng Dye, Purple (6X)
25
6X
Notes:
BclI-HF has the same specificity as BclI (
NEB
#R0160), but it has been engineered for reduced star activity.Cleaves to leave a 5? GATC extension which can be efficiently ligated to DNA fragments generated by BamHI, BglII, MboI, Sau3AI and BstYI.This enzyme is blocked by dam methylation. More information can be found at Dam-Dcm and CpG Methylation.Star activity may result from a glycerol concentration of >5%