商品编号


R0196S-2,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

20,000 units/ml


Size

2,000 units

商品信息

Description:
Product Source
An
E. coli
strain that carries the NciI gene from
Neisseria cinerea
(NRCC 31006).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
NciI produces DNA fragments that have a single-base 5??extension which are more difficult to ligate than blunt-ended fragments.Cleavage of mammalian genomic DNA is impaired by overlapping CpG methylation.For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch? PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.